Fig. 2: High matrix stiffness promotes the stemness of bladder cancer cells.
From: Matrix stiffness maintains bladder cancer stemness via integrin-nuclear skeleton axis
A, B Western blot analysis and quantification of stemness marker expression in T24 cells cultured on 5 kPa, 16 kPa and 30 kPa polyacrylamide gels. C Spheres of representative images of the indicated T24 and UM-UC-3 cells cultured on 5 kPa and 30 kPa polyacrylamide gels. (bar = 100 μm). D, E Histograms showing the mean numbers and diameters of cultured spheres. F Transwell Matrigel invasion assay of representative images of the indicated T24 and UM-UC-3 cells cultured on 5 kPa and 30 kPa polyacrylamide gels. (bar = 200 μm). G Quantitative analyses of cell invasion through Matrigel-coated membranes. H Colony formation assay of representative images of the indicated T24 and UM-UC-3 cells cultured on 5 kPa and 30 kPa polyacrylamide gels. I Quantitative analyses of the number of cell colonies. J Calcein-AM/PI staining of representative images of the indicated T24 and UM-UC-3 cells cultured on 5 kPa and 30 kPa polyacrylamide gels. (survival, green + ; dead, red + ). (bar = 100 μm). K Quantitative statistical graph of (J). Data are represented as mean ± SEM. In all bar graphs, each dot represents one biological replicate. *p < 0.05, **p < 0.01, ***p < 0.001, ns no significance by unpaired Student’s t test (D, E, G, I, K) or one-way ANOVA (B).
