Fig. 3: Cancer-associated adipocytes could provide triglycerides to tumor cells. | Cell Death & Disease

Fig. 3: Cancer-associated adipocytes could provide triglycerides to tumor cells.

From: Necrotic and apoptotic adipocytes in the hypoxic tumor microenvironment supply triglycerides to induce cisplatin resistance in the metastatic lymph nodes of head and neck carcinoma

Fig. 3

A Typical pictures (200×) and quantitative analysis of Oil red O-stained areas in adipocytes cocultured with Fadu cells for different periods (n = 3). B The changes of relative expression level of differentiation genes, lipogenic genes, fibroblast signature genes, and cancer-promoting cell factors related genes in adipocytes after coculture with Fadu and TU686 cells (n = 3). C Typical HE staining pictures of 2 patients’ metastatic neck nodes and negative neck nodes with adipocytes circled (left). Adipocytes’ diameter and area were calculated and compared (right). D Typical oil red O staining pictures of 2 patients’ metastatic neck nodes and negative neck nodes with lipid droplets’ area and intensity (Oil red O staining area/cell area) calculated. E The level of triglycerides in the supernatant of adipocytes during coculture with fadu and TU686 cells (n = 3). F Fadu and TU686 cells’ intracellular lipid droplets were stained using BODIPY 493/503 (left), and the changes of stained area after adding adipocytes’ conditioned medium were analyzed (right, n = 3). Green: BODIPY. Blue: DAPI. TG triglyceride. CM conditioned medium. MLN metastatic positive neck nodes. LN negative neck nodes. LD lipid droplets. means ± SEM. *P < 0.05, ‌**P < 0.01, ‌***P < 0.001, ****P < 0.0001, ns no statistical significance.

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