Fig. 4: Hypoxia tumor microenvironment causes adipocytes to necrosis and apoptosis. | Cell Death & Disease

Fig. 4: Hypoxia tumor microenvironment causes adipocytes to necrosis and apoptosis.

From: Necrotic and apoptotic adipocytes in the hypoxic tumor microenvironment supply triglycerides to induce cisplatin resistance in the metastatic lymph nodes of head and neck carcinoma

Fig. 4

A–C transcriptome sequencing result of hypoxia and normoxia Fudu cells. GSEA in the cytokine-cytokine receptor interaction KEGG pathway (A). STRING pathway enrichment relies on the KEGG database “environmental information processing” category (B). Volcano plots of differential genes (C). D Relative mRNA expression level of TNF superfamily. E Adipocyte nucleus was stained using Hoechst 33342 and PI, and the changes of apoptosis and necrosis cell rates in the hypoxia condition or after adding tumor cells’ conditioned medium were calculated. F Annexin/PI stain of adipocytes in the hypoxia condition or after adding tumor cells’ conditioned medium were analyzed using flow cytometry. OD oxygen deficiency. CM: conditioned medium. Blue: Hoechst 33342. Red: PI. n = 3, means ± SEM. *P < 0.05, ‌**P < 0.01, ‌***P < 0.001, ****P < 0.0001, ns no statistical significance.

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