Fig. 3: Chidamide relieved the suppressive effect of HDAC3 on the sensitivity of gastric cancer cells to 5-FU. | Cell Death & Disease

Fig. 3: Chidamide relieved the suppressive effect of HDAC3 on the sensitivity of gastric cancer cells to 5-FU.

From: Chidamide enhances the sensitivity of gastric cancer to 5-fluorouracil chemotherapy by suppressing the HDAC3/HNF4A/TYMS axis

Fig. 3: Chidamide relieved the suppressive effect of HDAC3 on the sensitivity of gastric cancer cells to 5-FU.

A GC cell lines, including MKN-45, HGC-27, were transfected with shNC, shHDAC3-1, shHDAC3-2, and shHDAC3-3, to construct HDAC3 knockdown cell lines. HDAC3 and TYMS expression was analyzed using Western blot. B–D GC cell lines, including MKN-45, HGC-27, were transfected with shNC or shHDAC3-2. B Cell viability was measured using the CCK-8 assay. C Cell proliferation was assessed via colony formation assay. D Sensitivity to 5-FU in GC cells was evaluated using the CCK-8 assay. All experiments were repeated at least three times, *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page