Fig. 5: CK-1α was identified as a novel cargo protein of SNX3 in pulmonary fibrosis.
From: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling
A Ven diagram revealed the quantity of SNX3-interacting proteins overlapped by the IP-MS database and proteomics analysis. B The protein mass spectrum of CK-1α were shown. C Fresh lung homogenates were sparked by anti-Flag or anti-CK-1α for CK-1α or SNX3 detection in co-IP analysis. D The protein level of CK-1α in mice were detected by IHC staining analysis (Scale bar: 200 μm; n = 8 mice). E The change colocalization of SNX3 with CK-1α in Snx3-cTg mice were measured by IF staining analysis; Scale bar: 100 μm, n = 8 mice. F The protein level of CK-1α in Snx3-cTg mice were detected by IHC staining analysis (Scale bar: 200 μm; n = 8 mice). G The change colocalization of SNX3 with CK-1α in Snx3-cKO mice was measured by IF staining analysis; Scale bar: 100 μm, n = 8 mice. H The protein level of CK-1α in Snx3-cKO mice were detected by IF staining analysis (Scale bar: 100 μm, n = 8 mice). I-J IF staining analysis were performed to detect the protein levels and colocalization of CK-1α with Rab 5a and PSMC3; Scale bar: 10 μm; n = 3 experiments. K The flow chart of CK-1α intracellular recycling and degradation. Were created with BioGDP.com The data were shown as means ± SEM. *P < 0.05 vs. CTL+ Saline group or sh-NC. #P < 0.05 vs CTL + BLM group or sh-NC + TGF-β1. ns, not significant.
