Fig. 6: SNX3/CK-1α axis induced Wnt/β-catenin signaling pathway activation and pulmonary fibrosis.
From: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling
A Snx3-cKO mice and CTL mice were administered AAV6-CK-1α (50 μL, i.p injection) and BLM (3 mg/kg, intratracheal instillation). B, C Minute respiratory volume and Peak inspiratory flow were detected by the EMKA system; n = 8 mice. D Representative images of hydroxyproline concentration were shown, n = 8 mice. E Representative images of IHC staining analysis of β-catenin in lung tissue sections as indicated (Scale bar: 200 μm; n = 8 mice). F, G H&E staining and PSR staining of lung tissues are shown; Scale bar: 200 μm, n = 8 mice. H The wound healing analysis detected the migratory ability of AT2 cells; Scale bar: 200 μm; n = 3 experiments. I The protein level and nuclear distribution of β-catenin were measured by IF staining analysis (Scale bar: 25 μm, n = 3 experiments). The data were shown as means ± SEM. *P < 0.05 vs. CTL+ Saline group or sh-NC. #P < 0.05 vs CTL + BLM group or sh-NC + TGF-β1. &P < 0.05 vs Snx3-cKO + BLM group or sh-SNX3 + TGF-β1. ns, not significant.
