Fig. 1: Suppression of body weight and peripheral blood in mice by DR post-irradiation.

A Experimental scheme. Wild-type C57BL/6 mice (2 months old) were irradiated with 4.5 Gy X-rays, fed with either AL diet or DR diet (daily food intake restricted to 70% of the intake of age- and sex-matched AL mice). NIR mice receiving an AL or DR diet were also monitored as a control (n = 5 mice per group from 1 experiment representative of 2 independent experiments). B Ratio of body weight of mice at the indicated time points to the average initial body weight post-irradiation and no irradiation. C–J PB cell counting at the indicated time points. Absolute counting value of WBC (C), Nitrification (E), Lymphocytes (G), RBC (I) and Platelet (J). D, F, H Ratio of the number of WBC (D), Neutrophil (F), Lymphocytes (H) in DR mice at each time point to the average number in AL mice. Spleen (K) and thymus weight (M) at the indicated time points. Ratio of spleen weight (L) and thymus weight (N) in DR mice at each time point to the average spleen or thymus weight in AL mice. Results were displayed as mean ± SD; ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by Two-way ANOVA test and multiple comparisons. AL ad libitum feeding, DR dietary restriction, NIR AL no irradiated ad libitum group, NIR DR no irradiated dietary restriction group, IR AL irradiated ad libitum group, IR DR irradiated dietary restriction group, WBC white blood cell, RBC red blood cell, d days, m months. The purple symbols indicate statistical significance between the non-irradiated groups. The green symbols indicate statistical significance between the irradiated groups.