Fig. 3: DR post-irradiation suppresses hematopoietic cell function in mice.

A Experimental scheme. Two-month-old wild-type C57BL/6 mice were subjected to 4.5 Gy X-ray irradiation and subsequently fed with an AL diet or DR diet for one month. Afterwards, 2 × 10⁶ BM cells from irradiated Ly5.2 donor mice were mixed with 1 × 10⁶ BM cells from Ly5.1/5.2 heterozygous competitor mice and primarily transplanted into lethally irradiated (9 Gy) Ly5.1 recipient mice for competitive BM transplantation. Four months after the primary transplantation, 10 × 10⁶ BM cells from the primary recipients were serially transplanted into lethally irradiated (9 Gy) Ly5.1 recipient mice. PB chimerism was analyzed at specified time points post-transplantation via FACS using blood collected from the retro-orbital sinus (n = 5 mice per group from 1 experiment representative of 2 independent experiments). B–I Chimerism of donor-derived cells in the peripheral blood, including WBCs (B), B cells (D), T cells (F), and myeloid cells (H), after primary transplantation at the indicated time points. Representative FACS plot of WBCs (C), B cells (E), T cells (G), and myeloid cells (I) 4 months after primary transplantation. Chimerism of donor-derived cells in the PB, including WBCs (J), B cells (L), T cells (N), and myeloid cells (P), after secondary transplantation at the indicated time points. Representative FACS plot of WBCs (K), B cells (M), T cells (O), and myeloid cells (Q) 4 months after secondary transplantation. Results were displayed as mean ± SD;. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by Two-way ANOVA test with Tukey’s multiple comparisons test. BMT: bone marrow transplantation.