Fig. 7: Suppression of hematopoiesis in mice by RF post-irradiation.

A Experimental scheme. Wild-type C57BL/6 mice (2 months old) were irradiated with 4.5 Gy X-rays, fed with either AL diet or DR diet or DR diet 1 week, then AL feeding 3 weeks (daily food intake restricted to 70% of the intake of age- and sex-matched AL mice). NIR mice receiving an AL or DR diet were also monitored as a control (n = 5 mice per group from 1 experiment representative of 2 independent experiments. B and C G6PD enzyme activity (B) and NADPH content (C) in BM cells of RF mice 1 month post-irradiation, analyzed using ELISA. WBC (D), Neutrophil (E), and lymphocyte (F), RBC (G), platelet (H) counts in PB of RF mice 1 month post-irradiation. I, J Spleen and thymus weights of RF mice 1 month post-irradiation. K Total BM cell counts of RF mice 1 month post-irradiation. FACS analysis of frequencies of B220⁺ lymphocytes in PB (L), spleen (M), BM (N); and frequencies of HSCs (O), CMPs (P) and GMPs (Q), MEPs (R), and RMBMs (S) of RF mice 1 month post-irradiation. T Apoptosis rates in HSCs of RF mice 1 month post-irradiation. U Average fluorescence intensity of ROS in BM cells of RF mice 1 month post-irradiation. V and W Quantification and representative images of γ-H2AX and 53BP1 colocalization in BM cells of RF mice 1 month post-irradiation, determined by immunofluorescent staining (scale bar: 10 μm). Results were displayed as mean ± SD; ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 by one-way ANOVA test with Tukey’s multiple comparisons test. IR RF: irradiated refeeding group.