Table 2 Key resources used in this study.
Reagent or resource | Source | Identifier |
|---|---|---|
Antibodies | ||
Rabbit polyclonal anti-SNAP23 (for WB, IP, IHC, IF) | Proteintech | Cat# 10825-1-AP, RRID:AB_2192022 |
TRIM21 (D1O1D) Rabbit mAb (for WB, IP) | CST | Cat# 92043,RRID:AB_2800177 |
TFAM (D5C8) Rabbit mAb (for WB, IP, IHC) | CST | Cat# 8076, RRID:AB_10949110 |
Ubiquitin (E6K4Y) XP® Rabbit mAb (for WB) | CST | Cat# 20326, RRID:AB_3064918 |
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (for WB) | CST | Cat# 5625, RRID:AB_10699459 |
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit (for WB) | CST | Cat# 9664, RRID:AB_2070042 |
Cleaved Caspase-9 (Asp330) (D2D4) Rabbit (for WB) | CST | Cat# 7237, RRID:AB_10895832 |
COX1 Rabbit mAb (for WB) | Abclonal | Cat# A23123, RRID:AB_3096107 |
ATP5A1 Rabbit mAb (for WB) | Abclonal | Cat# A11217, RRID:AB_2861524 |
ATP6V1E1 Rabbit pAb (for WB) | Abclonal | Cat# A3756, RRID:AB_2765253 |
SDHA Rabbit mAb (for WB) | Abclonal | Cat# A13852, RRID:AB_2861697 |
HA Tag Recombinant antibody (for IP, WB, CoIP) | Proteintech | Cat# 81290-1-RR,RRID:AB_2935602 |
DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (for IP, WB, CoIP) | Proteintech | Cat# 66008-4-Ig, RRID:AB_2918475 |
β-Actin mAb (for WB) | Proteintech | Cat# 66009-1-Ig, RRID:AB_2687938 |
Goat anti-Mouse IgG (H + L) Secondary Antibody, HRP | ThermoFisher | Cat# 31430; RRID: AB_228307 |
Goat anti-Rabbit IgG (H + L) Secondary Antibody, HRP | ThermoFisher | Cat# 31460; RRID: AB_228341 |
IRDye® 680RD Goat anti-Mouse IgG (H + L) Secondary Antibody | LI-COR Biosciences | Cat# 925-68070, RRID:AB_2651128 |
IRDye® 680RD Goat anti-Rabbit IgG (H + L) Secondary Antibody | LI-COR Biosciences | Cat# 925-68071, RRID:AB_2721181 |
IRDye® 800CW Goat anti-Mouse IgG (H + L) Secondary Antibody | LI-COR Biosciences | Cat# 925-32210, RRID:AB_2687825 |
IRDye® 800CW Goat anti-Rabbit IgG (H + L) Secondary Antibody | LI-COR Biosciences | Cat# 925-32211, RRID:AB_2651127 |
Biological samples | ||
Colorectal cancer biopsies sample | Shanghai General Hospital | N/A |
Tumor tissues of CRC patients | Shanghai General Hospital | N/A |
Chemicals | ||
DMEM Medium | Gibco | Cat# 61965059 |
Trypsin-EDTA (0.5%) | Gibco | Cat# 15400054 |
Opti-MEM I Reduced Serum Medium | Gibco | Cat# 31985047 |
Oxaliplatin | MCE | Cat# 61825-94-3 |
N-Acetyl-L-cysteine (NAC) | MCE | Cat# 616-91-1 |
Dimethyl sulfoxide | MCE | Cat# 67-68-5 |
Cycloheximide | MCE | Cat# 66-81-9 |
MG132 | MCE | Cat# 133407-82-6 |
TRIzol | ThermoFisher | Cat# 15596026 |
puromycin | Sigma-Aldrich | Cat# 58-58-2 |
G418 | Sigma-Aldrich | Cat# 108321-42-2 |
Penicilin/Streptomicin | NCM Biotech | Cat# C100C5 |
RIPA Buffer | NCM Biotech | Cat# WB3100 |
Universal Antibody Diluent | NCM Biotech | Cat# WB500D |
DAPI | Sigma-Aldrich | Cat# D9542-1 |
Critical commercial assays | ||
TUNEL BrightGreen Apoptosis Detection Kit | Vazyme | Cat# A112-03 |
Cell Counting Kit-8 | DOJINDO | Cat# CK04-100 |
CellTiter-Glo® 2.0 Cell Viability Assay | Promega | Cat# G9242 |
FITC Annexin V Apoptosis Detection Kit with PI | BioLegend | Cat# 640914 |
Caspase-3 Activity Assay Kit | Abcam | Cat# ab252897 |
Reactive Oxygen Species Assay Kit | Beyotime | Cat# S0033S |
Immunoprecipitation Kit with Protein A + G Agarose Gel | Beyotime | Cat# P2197M |
Seahorse XFp Cell Mito Stress Test Kit | Agilent Technologies | Cat# 103015-100 |
Oligonucleotides | ||
shSNAP23 | This paper | GCAGTATCCTGGGAAATCT |
SNAP23 (rSNAP23) | This paper | GAAGCATCTGGTAACCT |
shTrim21-1 | This paper | GGAAGTGGAAATTGCAATAAA |
shTrim21-2 | This paper | CTGCTGCAGGAGGTGATAATT |
TRIM21 | F :5’-CTCGGATCCGCCACCATGGCTTCAGCAGCACGCTT-3’ R :5’-CCCTCTAGACTCGAGATAGTCAGTGGATCCTTGTG-3 | |
TRIM21 (△1–81aa) | This paper | F :5’-CTCGGATCCGCCACCATGGAGGCCAGAGAGGGCAC-3’ R :5’-CCCTCTAGACTCGAGATAGTCAGTGGATCCTTGTG-3 |
TRIM21 (△1–128aa) | This paper | F :5’-CTCGGATCCGCCACCATGGAGGAGGCTGCACAGGA-3’ R :5’-CCCTCTAGACTCGAGATAGTCAGTGGATCCTTGTG-3 |
TRIM21 (△1–268aa) | This paper | F :5’-CTCGGATCCGCCACCATGCTCAGGAGTGTGTGCCA-3’ R :5’-CCCTCTAGACTCGAGATAGTCAGTGGATCCTTGTG-3 |
TRIM21 (△268–465aa) | This paper | F1 :5’-CTCGGATCCGCCACCATGGCTTCAGCAGCACGCTT-3’ R1 :5’-TCCAATATTTTCTGGAGAGGTAATATCCAG-3 F2 :TATTACCTCTCCAGAAAATATTGGAT R2 :5’-CCCTCTAGACTCGAGATAGTCAGTGGATCCTT-3 |
TFAM | This paper | F :5’-CTCGGATCCGCCACCATGGCGTTTCTCCGAAGCAT-3’ R :5’-CCCTCTAGACTCGAGACACTCCTCAGCACCATATT-3 |
Software and algorithms | ||
GraphPad PRISM 9 | GraphPad | |
ImageJ software | GitHub | |
FlowJo | BD Bioscience | https://www.bdbiosciences.com/zh-cn/products/software/flowjo-v10-software |
Image Studioâ„¢ Software | LI-COR Biosciences |
