Fig. 2: iRhom2 ablation improves cell recovery and ER stress in M1- macrophages during IRI. | Cell Death & Disease

Fig. 2: iRhom2 ablation improves cell recovery and ER stress in M1- macrophages during IRI.

From: iRhom2 regulates HMGB1 secretion to modulate inflammation and hepatocyte senescence in an in vitro model of ischemia-reperfusion injury

Fig. 2: iRhom2 ablation improves cell recovery and ER stress in M1- macrophages during IRI.

A Representative immunoblot and relative quantification (B–D) of iRhom2, GRP78, Pro-caspase3, cleaved-caspase 3, PARP, and cleaved-PARP, in NTC or iRhom2 KO THP-1 derived macrophages during in vitro IRI at different time points. Actin-β and Calnexin (CANX) were used as loading controls (n = 3). E, F ROS detection and relative quantification in NTC or iRhom2 KO THP-1 derived macrophages during in vitro IRI at different time points. B–D, F Multiple unpaired t tests (*p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001; ns not significant). Created in BioRender. Zito, G. (2025) https://BioRender.com/vuvm400.

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