Fig. 3: YBX1 promotes progression of ccRCC. | Cell Death & Disease

Fig. 3: YBX1 promotes progression of ccRCC.

From: YBX1 orchestrates LDHA-mediated metabolic reprogramming and NF-κB activation to drive clear cell renal cell carcinoma progression

Fig. 3: YBX1 promotes progression of ccRCC.

A, B Effects of YBX1 overexpression on the proliferation of ACHN and 786-O cells. A Colony formation assay. B CCK-8 cell proliferation assay. C, D Effects of YBX1 overexpression on the migration and invasion of ACHN and 786-O cells. C Transwell cell migration assay. D Transwell cell invasion assay. E Detection of bioluminescence intensity of 8 pairs of tumors in nude mice using an in vivo imaging system. F Quantification analysis of bioluminescence intensity at the site of renal orthotopic tumors. G Statistical analysis of in situ tumor weight, expressed as the weight of the left minus the corresponding right kidney of nude mice. H Immunohistochemical staining and quantification to detect protein expression of YBX1, LDHA and Ki-67 in tumor tissues from control and YBX1 knockdown groups in nude mice. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Unpaired two-sided Student’s t-test in A, C, D, F, G and H. Two-way ANOVA with correction for multiple comparisons in B. Data are presented as mean ± SD.

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