Fig. 2: Effect of CSNK1D on the proliferation and apoptosis potential of HNSCC cells in vitro. | Cell Death & Disease

Fig. 2: Effect of CSNK1D on the proliferation and apoptosis potential of HNSCC cells in vitro.

From: CSNK1D inhibition suppresses head and neck squamous cell carcinoma progression through SHH and PTCH1 pathway

Fig. 2: Effect of CSNK1D on the proliferation and apoptosis potential of HNSCC cells in vitro.

a The mRNA and protein expression of CSNK1D were in the five groups of HNSCC cell lines and HOK, as shown by qRT-PCR and Western blot analysis. HNSCC, Head and neck squamous cell carcinoma. b Western blot analysis of CSNK1D protein levels assessed in HN4 and CAL27 cells treated with si-CSNK1D or oe-CSNK1D. c Growth curves of CSNK1D-silencing or CSNK1D overexpression and control HN4 and CAL27 cells as measured by a CCK-8 assay, **P < 0.01, ***P < 0.001. d Representative images and quantitative analysis of anchor-dependent colony formation assays in HN4 and CAL27 cells, **P < 0.01, ***P < 0.001, error bar values represent the standard deviation (SD). e Apoptosis flow cytometry of si-CSNK1D or oe-CSNK1D and corresponding control HN4 and CAL27 cells (scale bar, 100 μm).

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