Fig. 2: Administration of gas plasma technology in combination with pPAMPs results in significantly increased tumor inhibition.
From: Gas plasma-induced bacterial PAMP release promotes skin cancer cell death
a representative image of gas plasma treatment; b metabolic activity of A375, SCC-25, and A431 cells 24 h post gas plasma treatment and calculated IC25 values; c metabolic activity of A375, SCC-25, and A431 cells 24 h, 48 h, and 72 h after treatment with three different dilutions of bacterial pPAMP-containing supernatants, statistical analysis was performed using two-way analysis of variance (ANOVA); d metabolic activity of A375, SCC-25 and A431 cells 24 h after gas plasma or PAMP treatment alone or combination treatment, statistical analysis was performed using one-way ANOVA; e representative flow cytometry contour plots of the viable cell population; f quantification of viable cells 24 h after gas plasma or PAMP treatment alone or combination treatment, statistical analysis was performed using two-way ANOVA; g representative flow cytometry histogram of DAPI intensity for cell cycle analysis; h G2/G1 phase ratio in A375, SCC-25 and A431 cells 24 h after gas plasma or PAMP treatment alone or combination treatment, statistical analysis was performed using one-way ANOVA. cPAMPs = PAMPs derived from untreated bacteria. ctrl = control. hPAMPs = PAMPs derived from heat-inactivated bacteria. LPS = lipopolysaccharide. PL = gas plasma. pPAMPs = PAMPs derived from gas plasma-exposed bacteria.
