Fig. 2: In vitro and in vivo synthetic lethality between EMP and ARID1A in CRC cells.
From: MAP4 phosphorylation induced by ARID1A loss sensitizes colorectal cancer cells to EMP
Representative images (A) and statistical analysis (B) of cell viability are shown. Scale bar, 200 μm, n = 3. C, D EMP (15 μM) selectively induced apoptosis in ARID1A-deficient CRC cells, n = 3. E Apoptosis-related proteins were determined by Western blotting. Representative images (F) and cell viability (G) of RKO ARID1A isogenic cell lines treated with EMP (20 μM) are shown. Scale bar, 200 μm, n = 3. H, I Re-introduction of ARID1A in RKO cells reversed EMP-induced apoptosis, n = 3. J Apoptosis-related proteins were examined by Western blotting. K Schematic representation of the mouse tumor xenograft model. Tumor growth curve (L, M) and tumor weight (N, O) of HCT116 ARID1A+/+ and HCT116 ARID1A−/− xenografts treated with vehicle or 20 mg/kg EMP are presented, n = 5. In all relevant panels, data are presented as mean ± SD, *P < 0.05, **P < 0.01, Student’s t test.
