Fig. 3: EMP destabilizes interphase microtubule dynamics of ARID1A-deficient CRC cells.
From: MAP4 phosphorylation induced by ARID1A loss sensitizes colorectal cancer cells to EMP
A, B Gene ontology (GO) enrichment analyses of transcriptome sequencing. Analyses of microtubule polymerization in HCT116 (C, D) and RKO (E, F) ARID1A isogenic cell lines, n = 3. Representative images of immunofluorescence staining (G) and analysis of microtubule polymerization index (H), Scale bar, 10 μm, n = 3. I–N Effect of EMP on the microtubule polymerization. Microtubule polymerization assay was performed to detect the expression levels of polymeric and soluble tubulin (I, K, M), and quantification of polymeric microtubules are shown (J, L, N). VNR (6 nM) and PTX (6 nM) were used as positive control, n = 3. Effect of EMP on the microtubule polymerization of RKO ARID1A isogenic cell lines. Microtubule polymerization (O) and quantification of polymeric microtubules (P) are shown, n = 3. In all relevant panels, data are presented as mean ± SEM. ns not significant, *P < 0.05, **P < 0.01, Student’s t test. P and S represent polymeric and soluble fractions of tubulin, respectively.
