Fig. 3: S. Typhimurium triggers neutrophil and macrophage cell death in zebrafish through its Type III Secretion System. | Cell Death & Disease

Fig. 3: S. Typhimurium triggers neutrophil and macrophage cell death in zebrafish through its Type III Secretion System.

From: Programmed cell death pathways coordinate neutrophil and macrophage clearance in zebrafish and are differentially exploited by Salmonella Typhimurium

Fig. 3

Wild-type 2 dpf embryos were infected in yolk sac (A, B, C) or in the otic vesicle with wild-type (WT) or the isogenic SPI-1/2 mutant (SPI-1/2) strain of ST (D, E, F), controls were injected with PBS. A Susceptibility to ST was evaluated until 5 dpi. B The transcript levels of the indicated genes were analyzed at 24 hpi by RT-qPCR. C Caspase-1 activity was determined at 24 hpi using a fluorogenic substrate. D–G Neutrophil and macrophage recruitment to otic vesicle or total count was followed up at 1, 3 and 6 hpi. Each dot represents one individual, and the means ± SEM for each group is also shown. P values were calculated using one-way analysis of variance (ANOVA) and Tukey multiple range test. ns not significant; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001. The regions of interest (ROI) used for quantification in all experiments are indicated in the representative images. Bars: 500 µm.

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