Fig. 5: S. Typhimurium induces necroptotic death of zebrafish neutrophils and macrophages. | Cell Death & Disease

Fig. 5: S. Typhimurium induces necroptotic death of zebrafish neutrophils and macrophages.

From: Programmed cell death pathways coordinate neutrophil and macrophage clearance in zebrafish and are differentially exploited by Salmonella Typhimurium

Fig. 5

Wild-type 2 dpf embryos were infected the otic vesicle with wild-type (WT) strain of ST (A-D), controls were injected with PBS. From 1 h before ST injection, embryos were treated with the necroptosis inhibitor (necrostatin-2) or DMSO. A–D Neutrophil and macrophage recruitment to otic vesicle or total count was followed up at 1, 3 and 6 hpi. Each dot represents one individual, and the means ± SEM for each group is also shown. P values were calculated using one-way analysis of variance (ANOVA) and Tukey multiple range test. ns, not significant; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001. The regions of interest (ROI) used for quantification in all experiments are indicated in the representative images. Bars: 500 µm.

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