Fig. 1: PRPF40B downregulation impairs proliferation, colony formation, and induces cell-cycle arrest in SH-SY5Y cells. | Cell Death & Disease

Fig. 1: PRPF40B downregulation impairs proliferation, colony formation, and induces cell-cycle arrest in SH-SY5Y cells.

From: Regulation of NTRK2 alternative splicing by PRPF40B controls neural differentiation and synaptic plasticity

Fig. 1: PRPF40B downregulation impairs proliferation, colony formation, and induces cell-cycle arrest in SH-SY5Y cells.

A The resazurin assay was used to measure cell viability of SH-SY5Y WT, G1, and G2 cells over five consecutive days. Inset: PRPF40B expression was assessed by Western blot analysis using specific antibodies. B Western blot (top) and quantification (bottom) analysis of Ki-67 expression in SH-SY5Y WT and G2 cells. C Colony formation assay. Representative images of colonies formed by SH-SY5Y WT and G2 cells after 7 days (left). The graphs (right) show relative differences in colony formation and cell growth in G2 compared to control cells. D Cell-cycle distribution of SH-SY5Y WT and G2 cells. The graph shows the percentage of cells in each phase of the cell cycle. E SH-SY5Y WT and G2 cells were analyzed by Annexin V-FITC/PI staining with flow cytometry. The lower-right quadrant represents early apoptotic cells, while the upper quadrants represent late apoptotic cells. Summary graphs of the flow cytometry results are shown at the bottom. Data represent the mean ± SEM from three independent experiments. Statistical significance: *p ≤ 0.05 and **p ≤ 0.01.

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