Fig. 2: PRPF40B depletion impairs cell migration and neuronal differentiation in NB cells.
A Representative images and graphical representation of wound-healing assays performed using SH-SY5Y WT and G2 cells. Data represent the mean ± SEM from three independent experiments. B The neuronal differentiation assay was performed using RA and RA + BDNF. Top panels: Immunofluorescence staining with dual labeling of cells was performed using antibodies directed against β-Tubulin III (green) and p-FAKY397 (red). β-Tubulin III indicates neuronal plasticity, while p-FAKY397 indicates migration, adhesion, and neuronal synaptogenesis. Bottom panels: Phase-contrast microscopy photographs of the culture plate during differentiation. Scale bars: 100 µm. C Graphical representation of the fluorescence intensity for each label, with the area indicating neuronal growth. Data represent the mean ± SEM from two independent experiments, each with n = 10 images. Statistical significance: *p ≤ 0.05, and ****p ≤ 0.0001.
