Fig. 3: Identification of differentially expressed genes after PRPF40B silencing in NB cells. | Cell Death & Disease

Fig. 3: Identification of differentially expressed genes after PRPF40B silencing in NB cells.

From: Regulation of NTRK2 alternative splicing by PRPF40B controls neural differentiation and synaptic plasticity

Fig. 3: Identification of differentially expressed genes after PRPF40B silencing in NB cells.

A PRPF40B expression in SH-SY5Y WT, scrambled (SCR), and G2 cells. Cell lysates were resolved by SDS-PAGE, and proteins were transferred to membranes for the detection of PRPF40B and GAPDH expression using specific antibodies. B Heat map representation of differentially expressed (DE) genes in WT, SCR, and G2 cells. The top row shows a dendrogram representing clustering information and similarity relationships among the cell lines. The left column of the heat map displays the gene clusters. The color intensity of the rows represents changes in gene expression: red indicates upregulated genes, and blue indicates downregulated genes. C The Venn diagram illustrates the overlap among all DE genes. A total of 2243 DE genes were identified, including 1504 upregulated genes (red) and 739 downregulated genes (green). D RNA-seq validation was performed using RT-qPCR for selected DE genes of interest. Data represent the mean ± SEM from three to eight independent experiments. Statistical significance: *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001.

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