Fig. 4: PRPF40B silencing alters alternative splicing patterns in neuronal cells. | Cell Death & Disease

Fig. 4: PRPF40B silencing alters alternative splicing patterns in neuronal cells.

From: Regulation of NTRK2 alternative splicing by PRPF40B controls neural differentiation and synaptic plasticity

Fig. 4: PRPF40B silencing alters alternative splicing patterns in neuronal cells.

A Venn diagram showing the overlap of alternative splicing (AS) events among G2/SCR, G2/WT, and SCR/WT comparisons. B Pie chart illustrating the types of AS events among the 233 DE events. C Proportion of PRPF40B-regulated AS events compared to annotated events in the reference FAST DB database. D Structural and sequence characteristics of downregulated exons, including exon size, strength of acceptor sites, distance between branch points and acceptor sites, and length of polypyrimidine tracts. E Validation of six selected alternative exon splicing events by RT-qPCR. The y-axis represents the expression of the transcript in G2 cells relative to that in WT cells, normalized to 1. Data represent the mean ± SEM from three to six independent experiments. Statistical significance: **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001.

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