Fig. 5: PRPF40B regulates key signaling pathways involved in neuronal differentiation and synaptic plasticity. | Cell Death & Disease

Fig. 5: PRPF40B regulates key signaling pathways involved in neuronal differentiation and synaptic plasticity.

From: Regulation of NTRK2 alternative splicing by PRPF40B controls neural differentiation and synaptic plasticity

Fig. 5: PRPF40B regulates key signaling pathways involved in neuronal differentiation and synaptic plasticity.

A Schematic representation of the BDNF signaling pathway. B and F Western blot analysis and quantification of MAPK and PI3K pathway components (ERK 1/2, p-ERK, AKT, p-AKT, and PI3K) in control, RA, and RA/BDNF-treated WT and G2 cell lines. C and G Western blot analysis and quantification of synaptogenesis-related proteins (N-cadherin, complexin 1/2, synaptophysin, and synapsin 1a/b) in control, RA, and RA/BDNF-treated WT and G2 cell lines. D and H Western blot analysis and quantification of proteins related to neuronal size and plasticity (β-Tubulin III and drebrin) in control, RA, and RA/BDNF-treated WT and G2 cell lines. E and I Western blot analysis and quantification of PRPF40B expression in control, RA, and RA/BDNF-treated WT and G2 cell lines. Data represent the mean ± SEM from three independent experiments. Statistical significance: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001. In the quantification graphs, black and white bars represent SH-SY5Y WT and G2 cells, respectively.

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