Fig. 5: Validating role of NSUN2 and its downstream molecules SOCS3 in the polarization of TAMs in tumor specimens of Bladder cancer patients.
From: NSUN2-mediated m5C modification of SOCS3 mRNA modulates macrophage polarization in bladder cancer
A–C After knocking down NSUN2 in TAMs of BCa patients, both WB, PCR and ELISA showed that siNSUN2 group showed up-regulation of M1-related genes and down-regulation of M2-related genes, while the afterward knock-down of SOCS3 rescued these changes. D Results of flow cytometry revealed a greater proportion of M1 and a lower proportion of M2 in the siNSUN2 group while results in siNSUN2-siSOCS3 group are similar to the NC group in BCa TAMs. E NSUN2 promoted the pro-tumor effects of M2 TAMs while inhibiting the anti-tumor effects of M1 TAMs, and SOCS3 rescued the above effects brought by NSUN2.
