Fig. 7: NSUN2 regulates the protein expression of SOCS3 by regulating the stability and nuclear export of SOCS3 mRNA.
From: NSUN2-mediated m5C modification of SOCS3 mRNA modulates macrophage polarization in bladder cancer
A A series of RIP assays confirmed that the interaction between SOCS3 mRNA and the m5C antibody relies on the presence of NSUN2. B, C The sequence and location of the predicted m5C sites in SOCS3 mRNA. D RNA pull-down assays revealed that site 1 is the m5C site of SOCS3 mRNA. E NSUN2 decreases the stability of SOCS3 mRNA. F SOCS3 mRNA in the cytoplasm was increased in the siNSUN2 group. G The sequence of the mutation SOCS3 mRNA. H RNA pull-down assays revealed that NSUN2 cannot bind to the mutation site. I The stability of SOCS3 mRNA increased after the mutation.
