Fig. 8: NSUN2-mediated SOCS3 mRNA methylation requires the assistance of the m5C reader YBX1 and the m5C eraser TET2.
From: NSUN2-mediated m5C modification of SOCS3 mRNA modulates macrophage polarization in bladder cancer
A RNA pull-down assays revealed that the m5C reader YBX1 and the m5C eraser TET2 can also be pulled down by the validated m5C sites 1 and 3. B RIP assays revealed that SOCS3 mRNA can be enriched by both YBX1 and TET2 antibodies. C Knockdown of YBX1 improved the stability of SOCS3 mRNA, whereas knockdown of TET2 decreased its stability. D, E Knockdown of YBX1 decreased the mRNA and protein expression of M2-related genes and increased the expression of M1-related genes. In addition, TET2 knockdown resulted in the opposite trend.
