Fig. 3: Single-cell and spatial analysis of IL7R-associated tumor-immune interactions in ovarian cancer. | Cell Death & Disease

Fig. 3: Single-cell and spatial analysis of IL7R-associated tumor-immune interactions in ovarian cancer.

From: IL7R remodels immunosuppression tumor microenvironment and promotes macrophage polarization by regulating NF-κB/CXCL1 axis in ovarian cancer

Fig. 3

A UMAP projection of integrated single-cell transcriptomic data from mouse ovarian cancer tissues (N = 1), identifying distinct cellular clusters; B distribution of cellular clusters across individual mouse ovarian cancer samples; C stacked bar plot showing the percentage of major cell types in Il7r-KO and Il7r-WT samples, P values were calculated via Fisher’s exact test; D consecutive sections of human ovarian cancer tissue microarray (TMA) analyzed by multiplex immunofluorescence: Section 1: IL7R (yellow) and epithelial marker PANCK (orange); Section 2: T cell marker CD8 (green); Section 3: Macrophage marker CD68 (red), immunosuppressive-polarized macrophage marker CD206 (pink), and B cell marker CD19 (light blue). Nuclei were counterstained with DAPI (blue). Left panel: Tumor tissue from an IL7R-high patient with sparse immune cell infiltration in the stroma. Right panel: Tumor tissue from an IL7R-low patient with abundant immune cell infiltration. Images acquired at ×20 magnification (scale bar: 20 µm); E circos plot depicting cell-cell interaction networks: Left: IL7R-KO group; Right: IL7R-WT group. Gray ribbons connect interacting cell types, with ribbon color intensity reflecting interaction strength (analyzed by the CellCall algorithm); F immune cell infiltration scores estimated by CIBERSORT in TCGA-OV samples, stratified based on IL7R expression (high vs. low); G HALO spatial analysis of CD68 co-expression-positive cells within a 100-µm radius of tumor cells in ovarian cancer TMA sections: Comparison between IL7R-high and IL7R-low groups.

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