Fig. 4: NAT10 positively regulates PIK3R2 expression in GBM cells. | Cell Death & Disease

Fig. 4: NAT10 positively regulates PIK3R2 expression in GBM cells.

From: NAT10-mediated N4-acetylcytidine (ac4C) modification of PIK3R2 mRNA promotes malignant progression of glioblastoma

Fig. 4

A, B Dot blot analysis of RNA ac4C levels following NAT10 knockdown in U87 and LN229 cells. C Retrieval and intersection of RIP-seq and acRIP-seq data from public databases for HeLa and BxPC-3 cells. D qPCR analysis of gene expression changes after NAT10 knockdown in U87 cells. E, F Changes in PIK3R2 expression levels assessed by qPCR following NAT10 knockdown. G Changes in PIK3R2 expression levels assessed by Western blot following NAT10 knockdown. H Schematic representation of the structure of the NAT10 protein, highlighting the RNA-binding region (purple box) and the N-acetyltransferase domain (blue box) within the truncated form of NAT10. I Western blotting was performed to detect the expression of PIK3R2 in HEK293T cells transfected with NAT10-WT, NAT10-ΔN-acetyltransferase, and NAT10-ΔRNA helicase. J The ac4C modification sites on PIK3R2 were predicted using the PACES website. K Mutant plasmids were constructed for RNA-Pull Down assays to verify specific binding sites, and FUS was selected as a positive reference and EEF1A1 as a negative reference. Error bars represent the mean ± SD. n = 3. ***p < 0.001, **p < 0.01, *p < 0.05. (Student’s t test).

Back to article page