Fig. 1: HSD reduces B16-OVA melanoma growth in skin and lung, but does not inhibit growth of Y-1 adrenal cancer cells. | Cell Death & Disease

Fig. 1: HSD reduces B16-OVA melanoma growth in skin and lung, but does not inhibit growth of Y-1 adrenal cancer cells.

From: High-salt diet induces immune-independent re-differentiation, metabolic shut down and cell cycle arrest of melanoma

Fig. 1

A Experimental set-up. C57BL/6 mice were fed a NSD or HSD for 7 days before injection of B16-OVA cells. Mice were kept on the diets for 14 days while tumor growth was assessed. B Representative images of skin tumors (upper panel) and lung metastasis (lower panel) of B16-OVA melanoma from two independent experiments. C Tumor volume of B16-OVA skin melanoma over the course of 14 days. n = ≥ 5 independent experiments with 5–10 mice/group each. 2-way-ANOVA, with Sidaks multiple comparison test. D Number of B16-OVA lung metastasis (manually counted). n = 2 independent experiments with 3–4 mice/group each. Linear mixed effect model with treatment (HSD vs. NSD) as fixed factor and experimental replicate as random factor. Data are presented as mean ± SEM. E Gene expression level Ovalbumin in whole lung tissue as readout for presence of cancer cells. n = 2 independent experiments with 3–4 mice/group each. Linear mixed effect model with treatment (HSD vs. NSD) as fixed factor and experimental replicate as random factor. Data are presented as mean ± SEM. F Volume of skin Y-1 tumors over the course of 14 days. n = 2 independent experiments with 5–10 mice/group each. Two-way ANOVA mixed-effect analysis comparing row means with Sidak’s multiple comparison test. G Cell cycle distribution skin B16-OVA cells ex vivo. n = 2 independent experiments with ≤10 mice/group each. Linear mixed effect model with treatment (HSD vs. NSD) as fixed factor and experimental replicate as random factor. Data are presented as mean ± SEM. H Protein level (z-score) of cell cycle regulators (mass spectrometry of B16-OVA skin tumor cells ex vivo). Two-way ANOVA mixed-effect analysis with uncorrected Fishers LSD test. Stars indicates significance p ≤ 0.01. I Apoptosis assay of B16-OVA skin tumor cells ex vivo. n = 1 experiment with 5 mice/group. Unpaired two-tailed t-test. Data are presented as mean ± SEM. All analyses were done after sacrificing the mice on day 14 after tumor injection.

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