Fig. 5: Hypoxia promotes metabolic rewiring in PECs, resulting in resistance to cysteine deprivation.
From: Transsulfuration metabolism is essential for ferroptosis resistance in quiescent endothelial cells
A Western blot analysis for HIF-1α and SCL7A11 in HUVECs exposed to hypoxia (1% O2) or normoxia (21% O2) for 48 and 72 h. ACTIN was used as loading control, and band intensities were normalized on control condition (Normoxia 24 h). Statistical significance is given by one sample t-test and reported as a p-value in the figure. B Cell viability assay using crystal violet staining on PECs exposed to hypoxia (1% O2) or normoxia (21% O2) for 48 h, incubated in control medium (200 μM cystine) or FMCys-Cys for 24 h in presence or absence of TSPi. Statistical significance is given by one sample t-test and reported as a p-value in the figure. C Western blot analysis for CBS, CTH, GPX4, HO-1 and FSP1 in PECs exposed to hypoxia (1% O2) or normoxia (21% O2) for 48 h, incubated in control medium (200 μM cystine, NT) or FMCys-Cys for 24 h. Statistical significance is given by one sample t-test and reported as a p-value in the figure.
