Fig. 6: NPM1 sumoylation is critical for its tumor-promoting function in LUAD.
From: SERPINB3 enhances NPM1 sumoylation via inhibiting SENP3’s activity and promotes lung tumorigenesis
A Western blot analysis of NPM1 and NPM1 mutants (K230R and K263R) sumoylation in PC9 cells. The ratio of SUMO2-NPM1/tubulin was indicated. B Western blot analysis of NPM1 and NPM1 mutant (K230R and K263R) expression in Beas2B cells. C Growth curve of Beas2B control, NPM1 and NPM1 mutants (K230R and K263R) overexpression cells. Seed 5×104 Beas2B cells and count for 3 days. Values are mean ± SD from three independent experiments, ***p < 0.001. Two way ANOVA test was utilized for multiple group comparison. D Western blot analysis of NPM1 and NPM1 mutants (K230R and K263R) expression in PC9 cells. E Growth curve of PC9 control, NPM1 and NPM1 mutants (K230R and K263R) overexpression cells. Seed 1.5×105 PC9 cells and count for 3 days. Values are mean ± SD from three independent experiments, ****p < 0.0001. Two way ANOVA test was utilized for multiple group comparison. F Xenograft tumors growing from indicated PC9 cell lines were isolated from nude mice after inoculation for 36 days. G Xenograft tumor volume curve of PC9 control, NPM1 and NPM1 mutants (K230R and K263R) overexpression cells. Values are mean ± SEM, N = 5 mice for each group, **p < 0.01, ***p < 0.001, ****p < 0.0001. Two way ANOVA test was utilized for multiple group comparison.
