Fig. 3: HAUSP binds to LZTS2 through its MATH domain.
From: SPOP and HAUSP bidirectionally regulate LZTS2 ubiquitination to modulate the Wnt pathway
A Immunoblots of immunoprecipitates (IP, top two panels) or whole cell extracts (WCE, bottom two panels) from 293 T cells expressing the indicated plasmids. Of note, Fg-HAUSP was able to pull down Myc-LZTS2. B Myc-LZTS2 could immunoprecipitate Fg-HAUSP. C Endogenous LZTS2 interacted with endogenous HAUSP in HCT-116 cells. D Endogenous HAUSP immunoprecipitated endogenous LZTS2 in HT-29 cells. E Schematic drawings show the domains in HAUSP and the truncated mutants used in the following studies. F Fg-HAUSP-N could, but Fg-HAUSP-M and Fg-HAUSP-C could not pull down Myc-LZTS2. G Compared with Fg-HAUSP, Fg-HAUSP-ΔMATH failed to interact with Myc-LZTS2. (H) Fg-HAUSP interacted with Myc-LZTS2-C, not Myc-LZTS2-N.
