Fig. 5: SPOP and HAUSP compete for binding LZTS2 to control its ubiquitination. | Cell Death & Disease

Fig. 5: SPOP and HAUSP compete for binding LZTS2 to control its ubiquitination.

From: SPOP and HAUSP bidirectionally regulate LZTS2 ubiquitination to modulate the Wnt pathway

Fig. 5

A Fg-HAUSP weakened the interaction between HA-SPOP and Myc-LZTS2. B HA-SPOP enabled to decrease the affinity of Fg-HAUSP to Myc-LZTS2. C 293 T cells were transfected with the indicated plasmids and treated with 25 μM of MG132 for 6 h before cell harvesting. HAUSP, but not HAUSP-CA, antagonized SPOP-induced ubiquitination of LZTS2. Actin acts as a loading control.

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