Fig. 4: Knockdown of USP8 leads to decrease Hippo signaling pathway activity in TNBC. | Cell Death & Disease

Fig. 4: Knockdown of USP8 leads to decrease Hippo signaling pathway activity in TNBC.

From: Positive feedback regulation between USP8 and Hippo/YAP axis drives triple-negative breast cancer progression

Fig. 4

A, B Western blot showed the expression of USP8 and YAP protein in BT549 or MDA-MB-231 cells treated with siControl or siUSP8. As an internal standard, β-actin was utilized. C–F RT-qPCR showed the mRNA expression levels of YAP and downstream target genes CTGF and CYR61 in BT549 or MDA-MB-231 cells treated with siControl or two independent siRNAs. G, H Luciferase reporter assay of TEAD transcriptional activity in BT549 or MDA-MB-231cells treated with siControl or siUSP8. I, J Western blot showed the expression of USP8 and YAP protein in BT549 or MDA-MB-231 cells treated with DMSO or different concentrations of DUB-IN-2. β-actin functioned as the internal control. K–N RT-qPCR showed the mRNA expression levels of YAP and downstream target genes CTGF and CYR61 in BT549 or MDA-MB-231 cells treated with DMSO or different concentrations of DUB-IN-2. O, P Luciferase reporter assay of TEAD transcriptional activity in BT549 or MDA-MB-231cells treated with DMSO or different concentrations of DUB-IN-2. All Data are shown as mean ± SD, N = 3. *P < 0.05; **P < 0.01; ***P < 0.001.

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