Fig. 7: ALDH3A2 blocks NRF2 nuclear translocation via downregulation of SLC47A1 in GC cells. | Cell Death & Disease

Fig. 7: ALDH3A2 blocks NRF2 nuclear translocation via downregulation of SLC47A1 in GC cells.

From: ALDH3A2 negatively orchestrates gastric cancer progression through a synergistic induction of ferroptosis and ferroptosis-driven macrophage reprogramming

Fig. 7: ALDH3A2 blocks NRF2 nuclear translocation via downregulation of SLC47A1 in GC cells.

MGC803 and HGC27 cells were transduced with either PLV-EF1a empty lentivirus as a control (Con), PLV-EF1a-ALDH3A2 alone, or PLV-EF1a-ALDH3A2 plus pLV-EF1a-SLC47A1. A Venn diagram analysis of ALDH3A2-interacting genes retrieved from the Cancer Cell Metabolism Gene Database (ccmGDB) across head and neck squamous cell carcinoma (HNSC), kidney renal clear cell carcinoma (KIRC), lung squamous cell carcinoma (LUSC), and uterine corpus endometrial carcinoma (UCEC). B, D qRT-PCR analysis of SLC47A1 mRNA expression in MGC803 and HGC27 cells (n = 4). C, E Immunoblot analysis of SLC47A1 protein expression in MGC803 and HGC27 cells. Numbers below bands indicate relative protein levels normalized to β-actin (n = 4). F, G Immunofluorescence staining of NRF2 (green) with DAPI nuclear counterstain (blue) in MGC803 and HGC27 cells. Merged images are shown, and quantification of the NRF2 signal is provided in the right panels (n = 4). Scale bar = 20 µm. H qRT-PCR analysis of mitochondrial unfolded protein response markers LONP1 and HSP60 in MGC803 and HGC27 cells (n = 4). I Immunoblot analysis of LONP1, HSP60, and GPX4 protein levels, with quantification normalized to β-actin (n = 4). J Measurement of MDA content (n = 4). Statistical differences were analyzed using one-way ANOVA. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

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