Fig. 6: Genetic ablation of INTS13 attenuates malignant phenotypes and induces apoptosis in primary cervical cancer cells.

Primary pCCa-1 human cervical cancer cells were established via lentiviral CRISPR/Cas9 transfection, incorporating Cas9-expressing construct plus either control sgRNA (“koC”) or INTS13-specific sgRNAs (koINTS13-sg1/koINTS13-sg2). Expression levels of relevant proteins were subsequently analyzed (A). These cells were then cultured under specified conditions to assess malignant phenotypes: cell viability (CCK-8 assay, B), cell proliferation (EdU-positive nuclei ratio, C), and in vitro cell migration and invasion (Transwell assays, D, E). Additionally, mitochondrial membrane potential was determined by JC-1 staining (F), and cell apoptosis by nuclear TUNEL staining (G). Data are presented as mean ± standard deviation (SD) with n = 5 biological replicates. Asterisks (*) indicate statistically significant differences (P < 0.05) compared to “koC” group. “n.s.” denotes no statistically significant difference (P > 0.05). The experiments were independently replicated five times with consistent results. Scale bar represents 100 μm.