Fig. 1: The protein phosphorylation level in colorectal cancer tisssues by phosphoproteomic analysis. | Cell Death & Disease

Fig. 1: The protein phosphorylation level in colorectal cancer tisssues by phosphoproteomic analysis.

From: EIF4B Ser93 phosphorylation by ERK2 promotes epithelial-mesenchymal transition to drive colorectal cancer metastasis

Fig. 1: The protein phosphorylation level in colorectal cancer tisssues by phosphoproteomic analysis.

A Schematic illustration of TMT-labeled quantitative phosphoproteomic used for the detection of differentially expressed phosphoproteins (DPPs) in CRC tissues compared to the normal tissues. B Volcano plot showed the phosphorylated alteration of DPPs in CRC tissues compared to the controls. The blue up triangle represents the phosphorylated alteration of eIF4B (Uniprot ID: P23588) at Ser93. C, D KEGG and GO analysis was conducted to investigate the signaling pathways and cell functions which phosphorylated alteration DPPs enriched on. E GSEA analysis showed the significant enrichment of EMT in proteins phosphorylation increasing group. F Heat map of all DPPs meeting∣log2(T/N)∣ > 1, ion Score>50 and site probability above 90. Data were presented as normalized expression values of 3 independent replicates. The color key represents the normalized values: blue (low) to red (high). G Mass spectrometry analysis of eIF4B tryptic peptides (Ser93-Lys113).

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