Fig. 3: eIF4B knockdown inhibited CRC cell proliferation, migration, and invasion,while its Ser93 phosphorylation promoted these abilities in vitro.

The following assays (A–E) were all conducted in the negative control (NC) and eIF4B knockdown cells. A CCK-8 assays were used to evaluate cells viability. B, C Colony formation assays were conducted to assess the proliferation capacity. Representative images and quantification of colonies were presented. D Transwell migration and invasion assays were conducted to evaluate cell migratory and invasive abilities. Representative images were presented with scale bar as 100 μm. E Wound‑healing assays were conducted to evaluate cell migratory abilities. Representative images were presented with scale bar as 200 μm. The assays of (F–J) were performed in eIF4B WT, eIF4B S93A, and eIF4B S93D CRC cells. F, G Colony formation assays were used to detect cell proliferation capacities. Representative images and quantification of colonies were presented. H CCK-8 assays were conducted to evaluate cell viabilities. I Transwell migration and invasion assays were conducted to evaluate the cell migratory and invasive abilities. Representative images were presented with scale bar as 100 μm. J Wound‑healing assays were conducted to evaluate cell migratory abilities. Representative images were presented with scale bar as 200 μm. Error bars represent mean±SEM from at least three independent experiments. P values were determined by two-sided Student t test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.