Fig. 5: eIF4B knockdown inhibited CRC growth and metastasis, while Ser93 phosphorylation promoted these processes in vivo.
A The subcutaneous tumors generated from shNC and sheIF4B CRC cells were dissected and photographed. B Tumor weight and volume were recorded and analyzed. C Immunohistochemistry was used to visualize and compare the protein levels of eIF4B, Ki67, Snai1, Vim and N-cad in tumors collected from the two groups. Scale bar: 100 μm. D The xenograft IHC staining score of eIF4B, Snai1, Vim and N-cad, and the staining positive rate of Ki67. E Schematic illustration of splenic injection-induced liver metastasis model in nude mice. F Twenty-eight days later, mice were sacrificed, and liver tissues were photographed and subjected to H&E staining. G The number of liver metastatic nodules and area were counted. H Lung tissues collected from lung metastasis model via tail vein injection in nude mice were photographed and subjected to H&E staining. I The number of lung metastatic nodules and area were counted. The eIF4B WT, eIF4B S93A, and eIF4B S93D CRC cells were subcutaneously injected into the abdominal flanks of nude mice, respectively. Gross images, tumor volumes and tumor weights of these three groups were shown in (J) and (K). L Immunohistochemistry was used to visualize and compare the protein levels of eIF4B, p-eIF4BSer93, Ki67, Snai1, Vim and N-cad in tumors collected from the three groups. Scale bar: 100 μm. M The tissues of liver metastasis model generated from the three groups were photographed and subjected to H&E staining. N The number of liver metastatic nodules and area were counted. O Lung tissues were collected, photographed, and subjected to H&E staining after the establishment of lung metastasis model. P The number of lung metastatic nodules and area were counted. All the data are presented as mean±SEM (n = 5). P values were determined by two-sided Student t test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
