Fig. 4: RIPK1 knockout suppresses glioma cell migration and invasion in vitro.
From: Necroptotic signaling orchestrates glioblastoma malignancy and potentiates temozolomide response
A Schematic overview of transwell-based migration and invasion assays. ECM extracellular matrix. B, C Transwell assays showing reduced migration and invasion in RIPK1−/− and MLKL−/− U251 cells compared to wild-type controls. Scale bar, 100 μm. D, E Rescue experiments using plasmid transfection in WT and RIPK1−/− U251 cells followed by transwell assays. Scale bar, 100 μm. F Immunoblot analysis of migration- and EMT-related proteins in WT, RIPK1-knockout (KO), and RIPK1-overexpressing (OE) U251 cells. G Quantification of protein expression in (F). H REACTOME pathway enrichment analysis of differentially expressed genes in RIPK1−/− U251 cells, highlighting alterations in signaling and migration-associated pathways. All experiments were performed in triplicate. Error bars represented means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns no significance.
