Fig. 3: Knockdown of IRX3 suppresses cell migration, invasion and Wnt/β-catenin signaling pathway in GBM. | Cell Death & Disease

Fig. 3: Knockdown of IRX3 suppresses cell migration, invasion and Wnt/β-catenin signaling pathway in GBM.

From: IRX3-CDK14 axis promotes glioblastoma progression by regulating LRP6-mediated canonical Wnt/β-catenin pathway

Fig. 3: Knockdown of IRX3 suppresses cell migration, invasion and Wnt/β-catenin signaling pathway in GBM.

A, B GSEA of CGGA glioma patient data showed significant enrichment of gene sets involved in the Wnt pathway with the abundant genes being upregulated (cutoff: median). C Immunoblotting analysis of IRX3 and β-catenin expressions in GBM cells with IRX3 knockdown or control. D qRT-PCR analysis of c-Myc, c-Jun, CCND1 and WISP1 mRNA levels in GBM cells with IRX3 knockdown or control. Data were analyzed with Student’s t test and shown as means and SD (n = 3), **p < 0.01. E Representative microscopical images of transwell assay that detected migration and invasion of GBM cells with IRX3 knockdown or control. F Immunoblotting analysis of the indicated proteins in GBM cells with IRX3 knockdown or control.

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