Fig. 1: Mosaic localization pattern of TET2 is observed in CRC tissues.

A The schematic diagram shows: samples of TET2 with “nucleus only” and “nucleus major” were categorized from the previously classified “High TET2 in the nucleus” samples; similarly, samples of TET2 with “cytoplasm only” and “cytoplasm major” were segmented from the earlier identified “High TET2 in the cytoplasm” samples. B Representative IHC images of TET2 and 5mC in serial sections for CRC samples categorized into different types. Note that “cytoplasm only” and “nucleus only” were already shown in our previous study. C Kaplan–Meier survival plots for the four indicated groups of all 263 patients. All 263 samples were classified with Duck’s (D) and TNM (E) staging to analyze the relationship between staging and the four indicated groups of samples. The percentage of “cytoplasm major” patients increased in the indicated Duke’s stage B (F) and T₄N₀M₀ stage (G). The distribution of cells with nuclear TET2 at the bottom and the top of the mucosa was summarized in the “cytoplasm major” (H) and the “nuclear major” (I) groups. J A schematic illustration of the cytoplasm-nucleus shuttling of TET2 localization during CRC progression. Additional statistical information was provided in Table S3.