Fig. 2: Lap2α knockout suppresses the growth of mammary gland tumors.

A IHC analysis of Lap2α expression in tumor tissues from mice carrying the transgene PyMT and normal mammary gland tissue. B Mammary gland tumor growth of Lap2α^+/+-1 (Lap2α^+/+;UbCre-ERT2) or Lap2α^+/+-2 (Lap2^flox/flox) and Lap2α^−/− (Lap2α^flox/flox;UbCre-ERT2) mice. Mice bearing PyMT tumors (n = 8) were injected with 4-OHT when tumor volume reached to 100 mm³ in size and the tumor growth was monitored for 40 days (one tumor in each mouse). Tumor size was normalized to the starting size of each tumor. C Kaplan–Meier overall survival analysis of Lap2α^+/+ and Lap2α^−/− mice bearing PyMT tumors (n = 8). D IHC analysis of Lap2α, Ki-67 and γH2AX in mammary gland tumors from Lap2α^+/+ and Lap2α^−/− mice. E Quantitative analysis of Ki-67⁺ area (n = 6) and γH2AX⁺ area (n = 6) in mammary gland tumors of Lap2α^+/+ and Lap2α^−/− mice. Data are mean ± SDs for (B) and (E). Statistical tests were performed by two-way ANOVA with Tukey’s multiple comparisons test (B), log-rank test (C) and one-way ANOVA with Dunnett’s multiple comparisons test (E). Scale bar, 100 m.