Fig. 6: SERPINB5 promotes cervical cancer cell proliferation, metastasis, and tumor growth in vitro and in vivo. | Cell Death & Disease

Fig. 6: SERPINB5 promotes cervical cancer cell proliferation, metastasis, and tumor growth in vitro and in vivo.

From: M5C-driven stabilization of SERPINB5 promotes cervical cancer progression and chemotherapy resistance

Fig. 6: SERPINB5 promotes cervical cancer cell proliferation, metastasis, and tumor growth in vitro and in vivo.The alternative text for this image may have been generated using AI.

A Representative immunofluorescence images showing EdU (green) and Hoechst (blue) staining in HeLa cells transduced with shNC, shSERPINB5 #1, or shSERPINB5 #2. Scale bars, 100 μm. B Quantification of EdU-positive cells. Data are presented as mean ± SD from three independent experiments (n = 3). C Representative images of crystal violet-stained HeLa cells in transwell migration and invasion assays following SERPINB5 knockdown. Scale bars, 100 μm. D Quantification of migrated and invaded cells (n = 3). Data are presented as mean ± SD. E Representative images of nude mice bearing subcutaneous tumors derived from HeLa cells transduced with shNC or shSERPINB5 (n = 6 per group). F Photographs of tumors excised at the endpoint (n = 6 per group). G Tumor volumes were measured at the indicated time points. Data represent mean ± SD (n = 6). H Final tumor weights at sacrifice. Data represent mean ± SD (n = 6). I H&E staining and IHC for SERPINB5 and Ki67 were performed on tumor sections from xenograft mice. Scale bars, 100 μm. Statistical significance was determined using the two-tailed unpaired t-test (B, D, H) and two-way ANOVA followed by multiple comparisons test (G). NS not significant for P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.

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