Fig. 7: SERPINB5 modulates microtubule dynamics and cell cycle progression to regulate microtubule-targeting chemotherapy response in cervical cancer. | Cell Death & Disease

Fig. 7: SERPINB5 modulates microtubule dynamics and cell cycle progression to regulate microtubule-targeting chemotherapy response in cervical cancer.

From: M5C-driven stabilization of SERPINB5 promotes cervical cancer progression and chemotherapy resistance

Fig. 7: SERPINB5 modulates microtubule dynamics and cell cycle progression to regulate microtubule-targeting chemotherapy response in cervical cancer.The alternative text for this image may have been generated using AI.

A GSEA analysis of transcriptomic data from SERPINB5-knockdown HeLa cells revealed significant downregulation of pathways related to microtubule cytoskeleton organization. B Functional enrichment analysis comparing SERPINB5+ and SERPINB5- cancer cells in scRNA-seq data. C PPI network visualization of the core module genes downregulated by shSERPINB5 and associated with SERPINB5⁺ cells. Nodes represent genes, and edges represent interactions. Colors indicate gene functions. D Dot plot showing the expression of selected genes in SERPINB5⁺ and SERPINB5- cells. The size of the dots represents the percentage of expressing cells, and the color represents the average expression. E Correlation between SERPINB5 mRNA expression and the expression of CENPE, KIF16B, KIF21A and CIT in TCGA cervical cancer dataset. F Violin plots showing the RNA expression of CCNB1 and CDK1 in SERPINB5⁺ and SERPINB5- cancer cells (Wilcoxon rank-sum test). G Representative IHC staining of CENPE in xenograft tumor sections from HeLa cells transduced with shNC or shSERPINB5. Scale bars, 100 μm. HeLa cells transduced with shNC or shSERPINB5 were treated with paclitaxel (H) or vincristine (I) at gradient concentrations for 48 h, and both cell viability and IC50 values were measured using CCK-8 assay (n= 3). WT HeLa cells or NSUN2–/– HeLa cells were treated with paclitaxel (J) or vincristine (K) for 48 h, and IC50 values were determined by CCK-8 assay (n = 3). NSUN2–/– HeLa cells ectopically expressing an empty vector or SERPINB5 were treated with paclitaxel (L) or vincristine (M) at gradient concentrations for 48 h, and cell viability as well as IC50 values were measured using CCK-8 assay (n = 3). N Representative IHC staining of SERPINB5 in tumor sections from paclitaxel-resistant and -sensitive tumors. Scale bars, 50 μm. Statistical significance was determined using two-way ANOVA followed by multiple comparisons test. Data are presented as mean ± SD from at least three independent experiments. NS not significant for P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page