Table 1 Composition of cell culture media.
Item | Volume (mL) |
|---|---|
Growth media 1 | |
Epithelial cell medium (ScienCell) | 500 |
FBS | 10% |
Epithelial cell growth supplement | 5 |
Antibiotic solution | 5 |
Growth media 2 | |
RPE basal media (Lonza) | 200 |
L-glutamine | 4 |
h-FGF-B | 1 |
FBS | 4% |
GA-1000 | 0.2 |
Maturation medium | |
RPE basal medium (Lonza) | 200 |
L-glutamine | 4 |
hFGF-B | 1 |
GA-1000 | 0.2 |
