Fig. 5: FNIP2 inhibition improves glycolysis and mitochondrial respiration in AT cells.

A Graph showing cell proliferation of CTRL and AT fibroblasts stably infected with shSCR or shFNIP2 lentivirus after plating for the indicated times and quantified by the cell viability assay CellTiter-Glo. Normalized results from three independent experiments are shown in relative luminescence units (RLU). Error bars show SD. Multiple t-tests were used to calculate significance between the indicated couples for each time point. **P < 0.001. The remaining comparisons show no significant difference. B Extracellular acidification rates (ECAR) for shSCR and shFNIP2 encoding CTRL and AT cells 24 h after plating. Analysis included CTRL cells exposed to high glucose and nutrient-starved CTRL cells. C Quantification of basal glycolysis in the indicated samples. D Assessment of glycolysis upon glucose stimulation in the same set of samples. E Evaluation of peak glycolysis capacity in the samples. F Oxygen consumption rates (OCR) in the cells detailed in (B). G Measurement of basal respiration in the indicated samples. H Determination of ATP production-linked respiration in the indicated samples. One-way ANOVA was used for statistical calculations for all graphs. For each fibroblast line, two independent Seahorse runs were performed on different days. Within each run, 7 wells per line were measured (technical replicates). Plots display all wells; overlaid symbols show plate-adjusted means with 95% CIs from a model that includes ‘run/plate’ as a factor. For each run/plate groups were compared using one-way ANOVA on normalized per-well values, reporting per-plate P values and effect sizes P values (****P < 0.0001; ***P < 0.001) report plate-adjusted comparisons with multiplicity control (Tukey). Exact n: 2 runs per line; 7 technical wells per run.