Fig. 3: UHRF1 promotes AR ubiquitination and consequently AR degradation. | Cell Death & Disease

Fig. 3: UHRF1 promotes AR ubiquitination and consequently AR degradation.

From: UHRF1 regulates AR ubiquitination to promote the loss of AR signaling and enzalutamide resistance in progression of prostate cancer

Fig. 3: UHRF1 promotes AR ubiquitination and consequently AR degradation.

A Western Blot analysis of AR and UHRF1 expression levels following UHRF1 knockdown in LNCaP-EnzR and C4-2-EnzR cells. B, C RT-qPCR results of AR, UHRF1, KLK3 mRNA levels following UHRF1 knockdown in LNCaP-EnzR and C4-2-EnzR. D, E Western blot analysis of AR expression levels following UHRF1 knockdown and MG132 treatment for 48 h in LNCaP-EnzR and C4-2-EnzR. F, G LNCaP and C4-2 cells were transfected with GFP-UHRF1, Flag-AR, and HA-Ub, and AR ubiquitination was examined using co-immunoprecipitation (Co-IP). H 293 T cells were transfected with UHRF1-H730A, Flag-AR, and HA-Ub, and ubiquitination of AR was examined via Co-IP. I–L LNCaP and C4-2 cells were transfected with the negative control or GFP-UHRF1 and subsequently treated with cycloheximide (CHX), and the decay of the AR protein over time was analyzed via Western blot. M 293 T cells were transfected with GFP-UHRF1 and/or Flag-AR, and the interaction between UHRF1 and AR was determined using Co-IP in 293 T cells. N The interaction between UHRF1 and AR was determined using Co-IP in LNCaP-EnzR cells. O Western Blot analysis of UHRF1 expression following transfection of negative control plasmid, GFP-UHRF1, or UHRF1-H730A in C4-2 cells. P–Q C4-2 cells were transfected with negative control or UHRF1-H730A and subsequently treated with CHX. Decay of the AR protein over time was analyzed by Western blot.

Back to article page