Fig. 2: Inflammatory stimulation reduces ANGPTL2 expression and induces pyroptotic signaling in macrophages in vitro. | Cell Death & Disease

Fig. 2: Inflammatory stimulation reduces ANGPTL2 expression and induces pyroptotic signaling in macrophages in vitro.

From: ANGPTL2 inhibits macrophage pyroptosis and alleviates rheumatoid arthritis progression by regulating mitophagy via IGFBP5

Fig. 2: Inflammatory stimulation reduces ANGPTL2 expression and induces pyroptotic signaling in macrophages in vitro.

A qPCR analysis of Angptl2, Nlrp3, Caspase-1, Gsdmd, and Il-1β expression in RAW264.7 cells stimulated with LPS alone or LPS + ATP. B ELISA quantification of IL-1β secretion in culture supernatants from RAW264.7 cells stimulated with LPS alone or LPS + ATP. C Time-course qPCR analysis of Angptl2, Nlrp3, Caspase-1, Gsdmd, and Il-1β mRNA expression in RAW264.7 cells primed with LPS for 0, 3, 6, 12, and 24 h, followed by 1-h ATP stimulation prior to RNA extraction. D Corresponding time-course Western blot analysis of ANGPTL2, NLRP3, GSDMD, Caspase-1, and IL-1β expression in RAW264.7 cells under identical conditions. E qPCR analysis of Angptl2, Nlrp3, Caspase-1, Gsdmd, and Il-1β expression in BMDMs stimulated with LPS alone or LPS + ATP. F ELISA quantification of IL-1β secretion in culture supernatants from BMDMs stimulated with LPS alone or LPS + ATP. G Time-course qPCR analysis of Angptl2, Nlrp3, Caspase-1, Gsdmd, and Il-1β mRNA expression in BMDMs primed with LPS for 0, 3, 6, 12, and 24 h, followed by 1-h ATP stimulation prior to RNA extraction. H Corresponding time-course Western blot analysis of ANGPTL2, NLRP3, GSDMD, Caspase-1, and IL-1β expression in BMDMs under identical conditions. *p < 0.05, **p < 0.01, ***p <0.001, ****p <0.0001 in the indicated groups.

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