Fig. 4: RTN3 promotes DHCR7 degradation through ubiquitination. | Cell Death & Disease

Fig. 4: RTN3 promotes DHCR7 degradation through ubiquitination.

From: Declined RTN3 stabilizes DHCR7 to induce cholesterol-dependent tumor progression and MEK inhibitors insensitivity in thyroid cancer

Fig. 4: RTN3 promotes DHCR7 degradation through ubiquitination.The alternative text for this image may have been generated using AI.

A WB analysis of DHCR7 expression levels after knockout, knockdown and overexpression of RTN3 in KTC-1 (left) and K1 (right) cells. B IHC analysis of the correlation between RTN3 and DHCR7 protein levels of thyroid cancer tissues. Upper: Representative staining results of low and high expression of RTN3 and DHCR7; scale bar, 100 μm. Lower: Correlation analysis showed negative relationship between RTN3 and DHCR7 proteins. C mRNA levels of DHCR7 in RTN3 knockout, knockdown and overexpression KTC-1 (left) and K1 (right) cells. ns: P ≥ 0.05. D WB analysis of DHCR7 and RTN3 levels after CHX treatment for indicated time in RTN3 knockout (upper) or overexpression (lower) cells. E WB analysis of the levels of RTN3 and DHCR7 after vehicle control or MG132 treatment (20 μg/mL, 8 h) in RTN3 knockout cells. F Cells with RTN3 knockout or overexpression were transfected with Ub-HA plasmids and treated with MG132 (20 μg/mL, 8 h) 64 h after transfection. IP and WB assays using indicated antibodies were used to analyze the ubiquitination of DHCR7.

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